The Netherlands international could miss up to seven matches across all competitions, including the Copa del Rey semi-final second leg against Atletico Madrid and the Champions Round of 16 tie.
The injury comes as a setback for manager Hansi Flick, who had just regained a nearly full squad with Pedri's return. De Jong's absence creates a significant midfield gap, forcing Flick to rely on players like Marc Bernal, Marc Casado, or Eric García.